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waveform analysis software  (Hamamatsu)

 
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    Hamamatsu waveform analysis software
    Waveform Analysis Software, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/waveform+analysis+software/waveform+analysis+software/pmc11143964-271-6-10
    Average 90 stars, based on 1 article reviews
    waveform analysis software - by Bioz Stars, 2026-10
    90/100 stars

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    Software:

    Article Title: hsa-miR-548v controls the viscoelastic properties of human cardiomyocytes and improves their relaxation rates
    Article Snippet: Calcium imaging was performed using the functional drug screen system (FDSS) from Hamamatsu for 2 minutes. .. Signals analysis was then performed using Waveform Analysis Software from Hamamatsu. .. After 7 days of culture, hiPSC-CMs were sequentially fixed between days 30 and 35 with 4% paraformaldehyde (PFA) (1573590, Electron Microscopy Sciences) for 10 minutes and then permeabilized and blocked with 0.5% Triton X-100 (T-8787, MilliporeSigma) and 2% BSA (001-000-162, Jackson ImmunoResearch) in PBS (blocking solution) for 1 hour.



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    Cardiac differentiation from hiPSCs (A) Schematic of the protocol for the differentiation of cardiomyocytes from hiPS cells with (RA (+)) or without RA (RA (−)) treatment (B) The ratio of cardiomyocytes quantified by immunostaining with cardiac troponin-T (cTnT). The proportion of cTnT positive cells was 93.91 ± 2.51% in CT (control hiPS-CMs), or 96.79 ± 1.81% in AM (RA-treated hiPS-CMs), respectively (P = N.S.). Error bars represent SD of the mean from the values of independent experiments. n = 3. N.S., not significant (C) Immunofluorescence analysis of the percentage of MLC2a (atrial isoform) and MLC2v (ventricular isoform) positive expression in the CT and AM. Error bars represent SD of the mean from the values of independent experiments. ** p < 0.01 for comparison with MLC 2a+/MLC <t>2v-cardiomyocyte</t> in CT, ## p < 0.01 for comparison with MLC 2a+/MLC 2v-cardiomyocyte in AM, n = 3.
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    Image Search Results


    Cardiac differentiation from hiPSCs (A) Schematic of the protocol for the differentiation of cardiomyocytes from hiPS cells with (RA (+)) or without RA (RA (−)) treatment (B) The ratio of cardiomyocytes quantified by immunostaining with cardiac troponin-T (cTnT). The proportion of cTnT positive cells was 93.91 ± 2.51% in CT (control hiPS-CMs), or 96.79 ± 1.81% in AM (RA-treated hiPS-CMs), respectively (P = N.S.). Error bars represent SD of the mean from the values of independent experiments. n = 3. N.S., not significant (C) Immunofluorescence analysis of the percentage of MLC2a (atrial isoform) and MLC2v (ventricular isoform) positive expression in the CT and AM. Error bars represent SD of the mean from the values of independent experiments. ** p < 0.01 for comparison with MLC 2a+/MLC 2v-cardiomyocyte in CT, ## p < 0.01 for comparison with MLC 2a+/MLC 2v-cardiomyocyte in AM, n = 3.

    Journal: Frontiers in Pharmacology

    Article Title: High-Throughput Drug Screening System Based on Human Induced Pluripotent Stem Cell-Derived Atrial Myocytes ∼ A Novel Platform to Detect Cardiac Toxicity for Atrial Arrhythmias

    doi: 10.3389/fphar.2021.680618

    Figure Lengend Snippet: Cardiac differentiation from hiPSCs (A) Schematic of the protocol for the differentiation of cardiomyocytes from hiPS cells with (RA (+)) or without RA (RA (−)) treatment (B) The ratio of cardiomyocytes quantified by immunostaining with cardiac troponin-T (cTnT). The proportion of cTnT positive cells was 93.91 ± 2.51% in CT (control hiPS-CMs), or 96.79 ± 1.81% in AM (RA-treated hiPS-CMs), respectively (P = N.S.). Error bars represent SD of the mean from the values of independent experiments. n = 3. N.S., not significant (C) Immunofluorescence analysis of the percentage of MLC2a (atrial isoform) and MLC2v (ventricular isoform) positive expression in the CT and AM. Error bars represent SD of the mean from the values of independent experiments. ** p < 0.01 for comparison with MLC 2a+/MLC 2v-cardiomyocyte in CT, ## p < 0.01 for comparison with MLC 2a+/MLC 2v-cardiomyocyte in AM, n = 3.

    Article Snippet: Recording data were analyzed using Waveform Analysis of Cardiomyocyte Software (Ver.1.2.1J, Hamamatsu Photonics K.K.).

    Techniques: Immunostaining, Control, Immunofluorescence, Expressing, Comparison